<?xml version="1.0" encoding="UTF-8"?>
<!DOCTYPE article PUBLIC "-//NLM//DTD JATS (Z39.96) Journal Publishing DTD v1.4 20241031//EN" "https://jats.nlm.nih.gov/publishing/1.4/JATS-journalpublishing1-4.dtd">
<article article-type="research-article" dtd-version="1.4" xmlns:xlink="http://www.w3.org/1999/xlink" xml:lang="en" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
  <front>
    <journal-meta>
      <journal-id journal-id-type="publisher-id">odmj</journal-id>
      <journal-title-group>
        <journal-title xml:lang="en">OncoDaily Medical Journal</journal-title>
        <abbrev-journal-title abbrev-type="publisher">ODMJ</abbrev-journal-title>
      </journal-title-group>
      <issn pub-type="epub">3067-6444</issn>
      <publisher>
        <publisher-name>OncoDaily Medical Journal</publisher-name>
        <publisher-loc>
          <country>US</country>
        </publisher-loc>
      </publisher>
      <self-uri xlink:href="https://oncodailyjournal.com/"/>
    </journal-meta>
    <article-meta>
      <article-id pub-id-type="publisher-id">5865</article-id>
      <article-id pub-id-type="doi">10.69690/ODMJ-001-1224-5865</article-id>
      <article-categories>
        <subj-group xml:lang="en" subj-group-type="heading">
          <subject>Original Research</subject>
        </subj-group>
      </article-categories>
      <title-group>
        <article-title xml:lang="en">Evaluation of Virus-specific T-lymphocytes in CD45RA-depleted Products before and after Long-term Cryopreservation: Impact of Cell Resting and Storage Duration</article-title>
      </title-group>
      <contrib-group content-type="author">
        <contrib contrib-type="author" corresp="yes">
          <name name-style="western">
            <surname>Kazmina</surname>
            <given-names>Irina</given-names>
          </name>
          <aff xml:lang="en">Dmitry Rogachev National Medical Research Center of Pediatric Hematology, Oncology and Immunology, Moscow, Russia</aff>
          <email>rinakazmina@gmail.com</email>
        </contrib>
        <contrib contrib-type="author">
          <name name-style="western">
            <surname>Osipova</surname>
            <given-names>Elena</given-names>
          </name>
          <aff xml:lang="en">Dmitry Rogachev National Medical Research Center of Pediatric Hematology, Oncology and Immunology, Moscow, Russia</aff>
        </contrib>
        <contrib contrib-type="author">
          <name name-style="western">
            <surname>Melkova</surname>
            <given-names>Anastasia</given-names>
          </name>
          <aff xml:lang="en">Dmitry Rogachev National Medical Research Center of Pediatric Hematology, Oncology and Immunology, Moscow, Russia</aff>
        </contrib>
        <contrib contrib-type="author">
          <name name-style="western">
            <surname>Muzalevsky</surname>
            <given-names>Yakov</given-names>
          </name>
          <aff xml:lang="en">Dmitry Rogachev National Medical Research Center of Pediatric Hematology, Oncology and Immunology, Moscow, Russia</aff>
        </contrib>
        <contrib contrib-type="author">
          <name name-style="western">
            <surname>Efimenko</surname>
            <given-names>Maria</given-names>
          </name>
          <aff xml:lang="en">Dmitry Rogachev National Medical Research Center of Pediatric Hematology, Oncology and Immunology, Moscow, Russia</aff>
        </contrib>
        <contrib contrib-type="author">
          <name name-style="western">
            <surname>Levin</surname>
            <given-names>Pavel</given-names>
          </name>
          <aff xml:lang="en">Dmitry Rogachev National Medical Research Center of Pediatric Hematology, Oncology and Immunology, Moscow, Russia</aff>
        </contrib>
        <contrib contrib-type="author">
          <name name-style="western">
            <surname>Trahtman</surname>
            <given-names>Pavel</given-names>
          </name>
          <aff xml:lang="en">Dmitry Rogachev National Medical Research Center of Pediatric Hematology, Oncology and Immunology, Moscow, Russia</aff>
        </contrib>
        <contrib contrib-type="author">
          <name name-style="western">
            <surname>Maschan</surname>
            <given-names>Michael</given-names>
          </name>
          <aff xml:lang="en">Dmitry Rogachev National Medical Research Center of Pediatric Hematology, Oncology and Immunology, Moscow, Russia</aff>
        </contrib>
      </contrib-group>
      <pub-date date-type="pub" publication-format="electronic">
        <day>24</day>
        <month>12</month>
        <year>2025</year>
      </pub-date>
      <elocation-id>ODMJ-001-1224-5865</elocation-id>
      <permissions>
        <copyright-statement>Copyright (c) 2025 OncoDaily Medical Journal</copyright-statement>
        <copyright-year>2025</copyright-year>
        <copyright-holder>OncoDaily Medical Journal</copyright-holder>
      </permissions>
      <self-uri xlink:href="https://oncodailyjournal.com/10.69690/odmj-001-1224-5865/"/>
      <kwd-group xml:lang="en">
        <kwd>CD45RA-depletion</kwd>
        <kwd>cryopreservation</kwd>
        <kwd>ELISpot</kwd>
        <kwd>hematopoietic stem cell transplantation</kwd>
      </kwd-group>
      <custom-meta-group>
        <custom-meta>
          <meta-name>production-ready-file-url</meta-name>
          <meta-value>
            <ext-link ext-link-type="uri" xlink:href="https://oncodailyjournal.com/pub/uploads/2025/11/Evaluation-of-Virus.pdf"/>
          </meta-value>
        </custom-meta>
      </custom-meta-group>
    </article-meta>
  </front>
  <body/>
  <back>
    <ref-list>
      <ref id="R1">
        <mixed-citation>Leen AM, Bollard CM, Mendizabal AM, et al. Multicenter study of banked third-party virus-specific T cells to treat severe viral infections after hematopoietic stem cell transplantation. Blood. 2013 Jun 27;121(26):5113–23.</mixed-citation>
      </ref>
      <ref id="R2">
        <mixed-citation>Castagna L, Valli V, Timofeeva I, et al. Feasibility and efficacy of CD45RA+ depleted donor lymphocytes infusion after haploidentical transplantation with post-transplantation cyclophosphamide in patients with hematological malignancies. Transplant Cell Ther. 2021 Jun;27(6):478.e1–5.</mixed-citation>
      </ref>
      <ref id="R3">
        <mixed-citation>Hont AB, Powell AB, Sohai DK, et al. The generation and application of antigen-specific T cell therapies for cancer and viral-associated disease. Mol Ther. 2022 Jun 1;30(6):2130–52.</mixed-citation>
      </ref>
      <ref id="R4">
        <mixed-citation>Shook DR, Triplett BM, Eldridge PW, et al. Haploidentical stem cell transplantation augmented by CD45RA negative lymphocytes provides rapid engraftment and excellent tolerability. Pediatr Blood Cancer. 2015 Apr;62(4):666-73.</mixed-citation>
      </ref>
      <ref id="R5">
        <mixed-citation>Gasior Kabat M, Bueno D, Sisinni L, et al. Selective T-cell depletion targeting CD45RA as a novel approach for HLA-mismatched hematopoietic stem cell transplantation in pediatric nonmalignant hematological diseases. Int J Hematol. 2021 Jul;114(1):116-23</mixed-citation>
      </ref>
      <ref id="R6">
        <mixed-citation>Maschan M, Blagov S, Shelikhova L, et al. Low-dose donor memory T-cell infusion after TCR alpha/beta depleted unrelated and haploidentical transplantation: results of a pilot trial. Bone Marrow Transplant. 2018 Mar;53(3):264-73.</mixed-citation>
      </ref>
      <ref id="R7">
        <mixed-citation>Dunaikina M, Zhekhovtsova Z, Shelikhova L, et al. Safety and efficacy of the low-dose memory (CD45RA-depleted) donor lymphocyte infusion in recipients of αβ T cell-depleted haploidentical grafts: results of a prospective randomized trial in high-risk childhood leukemia. Bone Marrow Transplant. 2021 Jul;56(7):1614-24.</mixed-citation>
      </ref>
      <ref id="R8">
        <mixed-citation>Triplett BM, Muller B, Kang G, et al. Selective T-cell depletion targeting CD45RA reduces viremia and enhances early T-cell recovery compared with CD3-targeted T-cell depletion. Transpl Infect Dis. 2018 Feb;20(1):e12823.</mixed-citation>
      </ref>
      <ref id="R9">
        <mixed-citation>Bonifacius A, Lamottke B, Tischer-Zimmermann S, et al. Patient-tailored adoptive immunotherapy with EBV-specific T cells from related and unrelated donors. J Clin Invest. 2023 Jun 15;133(12):e163548.</mixed-citation>
      </ref>
      <ref id="R10">
        <mixed-citation>Zhang J, Yin Z, Liang Z, et al. Impacts of cryopreservation on phenotype and functionality of mononuclear cells in peripheral blood and ascites. J Transl Int Med. 2024 Mar 21;12(1):51-63.</mixed-citation>
      </ref>
      <ref id="R11">
        <mixed-citation>Luo Y, Wang P, Liu H, et al. The state of T cells before cryopreservation: effects on post-thaw proliferation and function. Cryobiology. 2017 Dec;79:65-70.</mixed-citation>
      </ref>
      <ref id="R12">
        <mixed-citation>Sunami K, Teshima T, Nawa Y, et al. Administration of granulocyte colony-stimulating factor induces hyporesponsiveness to lipopolysaccharide and impairs antigen-presenting function of peripheral blood monocytes. Exp Hematol. 2001 Sep;29(9):1117-24.</mixed-citation>
      </ref>
      <ref id="R13">
        <mixed-citation>Kuerten S, Batoulis H, Recks MS, et al. Resting of cryopreserved PBMC does not generally benefit the performance of antigen-specific T cell ELISPOT assays. Cells. 2012 Jul 30;1(3):409-27.</mixed-citation>
      </ref>
      <ref id="R14">
        <mixed-citation>Devadas SK, Khairnar M, Hiregoudar SS, et al. Is long term storage of cryopreserved stem cells for hematopoietic stem cell transplantation a worthwhile exercise in developing countries? Blood Res. 2017 Dec;52(4):307-10.</mixed-citation>
      </ref>
      <ref id="R15">
        <mixed-citation>Triplett BM, Shook DR, Eldridge P, et al. Rapid memory T-cell reconstitution recapitulating CD45RA-depleted haploidentical transplant graft content in patients with hematologic malignancies. Bone Marrow Transplant. 2015 Jul;50(7):968-77.</mixed-citation>
      </ref>
      <ref id="R16">
        <mixed-citation>Park HJ, Hong KT, Yun SO, et al. Successful treatment of refractory CMV colitis after haploidentical HSCT with post-transplant cyclophosphamide using CD45RA+ depleted donor lymphocyte infusion. Bone Marrow Transplant. 2020 Aug;55(8):1674-6.</mixed-citation>
      </ref>
    </ref-list>
  </back>
</article>
